Agilent webinář: An Introduction to Ion-Paired Reverse-Phase Oligonucleotide Separations: From Analysis to Purification

With the worldwide growth in the oligonucleotide markets, applying the appropriate tools for oligonucleotide analysis and purification is essential. Ion-paired reverse-phase (IP-RP) liquid chromatography involves the use of ion-pairing reagents to form neutral, hydrophobic complexes with charged oligonucleotides, facilitating their retention and separation on reversed phase columns.

This presentation introduces the principles and applications of IP-RP in oligonucleotide analysis. It will cover key factors influencing the efficiency of IP-RP methods such as column chemistry, choice of ion-pairing reagent, and mobile phase composition. Additionally, we will discuss method considerations for scaling up your oligonucleotide purification method.